Coding
Part:BBa_K3599035
Designed by: Zijia Liu Group: iGEM20_TPR_China (2020-10-24)
baPAD
an enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of baPAD was converting 4-coumaric acid into 4-hydroxystyrene. The codon usage was optimized for expression in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 220
- 1000COMPATIBLE WITH RFC[1000]
Reference
Kang, S. Y., Choi, O., Lee, J. K., Ahn, J. O., Ahn, J. S., Hwang, B. Y., & Hong, Y. S. (2015). Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain. Microbial cell factories, 14(1), 1-11. 10.1186/s12934-015-0268-7
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Categories
Parameters
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